Figure 4.

(A) 5′Cl5'd-(±)-ENBA reduces microglial activation in the dorsal horn of SNI mice. SNI induction increased the number of activated microglia, estimated by ratio processes length/soma diameter. (B) Iba-1 immunoreactivity (Iba-1-ir) in the ipsilateral dorsal horn 7 days post-SNI and after 5′Cl5′d-(±)-ENBA treatment. Quantitative analysis of spinal cord sections shows significantly increased numbers of activated microglia cells in the ipsilateral dorsal horn of SNI mice as compared with sham-operated mice. Chronic 5′Cl5′d-(±)-ENBA treatment reduced the number of activated microglia as compared with SNI mice. Data represent mean ± S.E.M., n = 3 mice per group. ° p < 0.05 vs. sham mice, * p < 0.05 vs. SNI mice. ANOVA, post hoc Tukey. Scale bars = 100 μm.