Figure 1.

HPLC chromatogram of bifendate and detection of bifendate after extraction by hepatocytes. (A) Bifendate. (B) Final washing eluate (lower line) and desorption eluate (upper line) of the control group. There was no detectable bifendate at 6.3 min in the eluate. (C) Final washing eluate (lower line) and desorption eluate (upper line) of the medicated group. No detectable bifendate at 6.3 min was found in the final washing eluate. Bifendate was detected at 6.3 min in the desorption eluate which indicated that bifendate could bind to hepatocytes. (D) Washing eluate after four washes (lower line) and eight washes (upper line). Bifendate was detected after four washes while it was almost eliminated after eight washes.