Figure 5.

T7 transcription mediated by the Ds–Pa pair from DNA templates containing Ds, with different lengths after the unnatural base position. (a) Sequences of templates used in this study. (b) Gel electrophoresis of transcripts from different length templates with natural NTPs (1 mM) and in the presence or absence of PaTP (1 mM). Transcripts were labeled at their 5′-termini with [γ-³²P]GTP. The positions of the full-length products are indicated by red arrows. The relative yield of each full-length transcript was determined by comparison to the yield of the native transcript from the template consisting of the natural bases only. The experiments were independently repeated three times, and representative data are shown.