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Binding capacity of intact THP and enzyme-digested products with TNF-α, was detected by Western blotting. (A) The molecular size of intact THP, TNF-α, and the different enzyme-digested THP products was identified by Coomasie blue staining. (B) The binding capacity of intact THP and enzyme-digested THP products was detected by Western blot using TNF-α (1° binder) and HRP-anti-TNF-α antibody (2° binder) as indicators.
