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1H-NMR stacked spectra of 3.0 mM analogue 3 in D2O. (a) 3.0 mM 2'-H-endo-S-c-di-GMP, 100 mM KCl, 60 °C. (b) 3.0 mM 2'-H-endo-S-c-di-GMP, 100 mM KCl, 30 °C. (c) 3.0 mM 2'-H-endo-S-c-di-GMP, no metal cation, 30 °C. (d) 3.0 mM 2'-H-endo-S-c-di-GMP, T1/T2 relaxation analysis (from DOSY experiments).
