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. 2012 Nov 9;17(11):13376–13389. doi: 10.3390/molecules171113376

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© 2012 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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¹H-NMR stacked spectra of 3.0 mM analogue 4 in D₂O. (a) 3.0 mM 2'-OMe-endo-S-c-di-GMP, 100 mM KCl, 60 °C. (b) 3.0 mM 2'-OMe-endo-S-c-di-GMP, 100 mM KCl, 30 °C. (c) 3.0 mM 2'-OMe-endo-S-c-di-GMP, no metal cation, 30 °C. (d) 3.0 mM 2'-OMe-endo-S-c-di-GMP, T₁/T₂ relaxation analysis (from DOSY experiments).