Fig. 1.

Setup of the custom-built slide scanner. A: Depicted is the technical setup, comprised of a microscope with a high-precision stage and a camera, a stage control unit and a PC workstation for remote control of the stage, image capturing and image processing. In order to fit large glass slides, a custom-built slide holder was added on top of the stage (image bottom left). B: The scanning approach with multiple overlapping images. C: The correction of the original image using a brightfield and a background image.