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. Author manuscript; available in PMC: 2018 Nov 30.
Published in final edited form as: Cell Rep. 2018 Jul 10;24(2):453–462. doi: 10.1016/j.celrep.2018.06.021

Figure 6. Pairing Alignments of A•A′, A•G′, and G•A′ Non-canonical Pairs within the Seed Segment in the Complex of RsAgo with gRNA and tDNA.

Figure 6.

(A) Sequence alignment of the gRNA-tDNA showing the position of the A3•A3′ non-canonical pair.

(B) Pairing alignment of the cis Watson-Crick A3(anti)•Hoogsteen A3′(syn) pair in the ternary complex. The 2Fo-Fc omit electron density map is contoured at 1.0σ.

(C) Sequence alignment of the gRNA-tDNA showing the position of the A8•A8′ non-canonical pair.

(D) Pairing alignment of the cis Watson-Crick A8(anti)•Hoogsteen A8′(syn) pair in the ternary complex. The 2Fo-Fc omit electron density map is contoured at 1.0σ.

(E) Sequence alignment of the gRNA-tDNA showing the position of the A8•G8′ non-canonical pair.

(F) Pairing alignment of the cis Watson-Crick A8(anti)•Hoogsteen G8′(syn) pair in the ternary complex. The 2Fo-Fc omit electron density map is contoured at 1.0σ.

(G) Sequence alignment of the gRNA-tDNA showing the position of the G8•A8′ non-canonical pair.

(H) Pairing alignment of the cis Watson-Crick G8(anti)•Hoogsteen A8′(syn) pair in the ternary complex. The 2Fo-Fc omit electron density map is contoured at 1.0σ.