Figure 3:

Intraperitoneal and subcutaneous B16-OVA tumors grown in C57BL/6 mice were prepared for flow cytometry, as outlined in Sections 3.1 and 3.4. Endothelial cells were gated as live singlet CD45^neg Ter119^neg CD31^pos. Fibroblasts are gated as live singlet CD45^neg Ter119^neg Podoplanin^pos. B cells are gated as live singlet CD45^pos CD3^neg CD19^pos. Abbreviations: S.C., subcutaneous; I.P., intraperitoneal. Error bars represent mean ± SEM. ns: P>0.05, *P<0.05, **P<0.01, ***P<0.001, ****P,0.0001. P values were calculated using the Mann-Whitney test.