Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (M musculus) |
RMA/S | 10.1002/ijc. 2910470711 |
RRID:CVCL_2180 | |
| Cell line (M musculus) |
MCA-205 | PMID: 2303716 | MCA-205 | |
| Cell line (M musculus) |
MCA205∆Tap2 | this paper | Generated using CRISPR/Cas9 described in materials/methods |
|
| Recombinant DNA reagent |
PresentER- SIINFEKL |
this paper | Generated using procedure described in materials/methods |
|
| Recombinant DNA reagent |
PresentER- FEKIILSN |
this paper | Generated using procedure described in materials/methods |
|
| Recombinant DNA reagent |
PresentER- MSIIFFLPL |
this paper | Generated using procedure described in materials/methods |
|
| Recombinant DNA reagent |
PresentER- SNFVSAGI |
this paper | Generated using procedure described in materials/methods |
|
| Recombinant DNA reagent |
PresentER- SNFVFAGI |
this paper | Generated using procedure described in materials/methods |
|
| Recombinant DNA reagent |
PresentER- SIYRYYGL |
this paper | Generated using procedure described in materials/methods |
|
| Recombinant DNA reagent |
PresentER- EQYKFYSV |
this paper | Generated using procedure described in materials/methods |
|
| Recombinant DNA reagent |
PresentER- TWHRYHLL |
this paper | Generated using procedure described in materials/methods |
|
| Recombinant DNA reagent |
PresentER- TAYRYHLL |
this paper | Generated using procedure described in materials/methods |
|
| Recombinant DNA reagent |
LentiCRISPRv2 | Addgene | Addgene:52961 | |
| Strain, strain background (m musculus) |
C57BL6/N | Envigo | Envigo:44 | |
| Strain, strain background (m musculus) |
B6.SJL- Ptprca/BoyAiTac |
Taconic Biosciences |
Taconic:4007 F | |
| Strain, strain background (m musculus) |
B6.129S6- Rag2tm1Fwa N12 |
Taconic Biosciences |
Taconic:RAGN12-F | |
| Antibody | anti-H-2Kb | Biolegend | Biolegend:116517 | used at 1:200 |
| Antibody | anti-SIINFEKL /H2kb |
Biolegend | Biolegend:141605 | used at 1:200 |
| Antibody | anti-CD45.2 | eBioscience | eBioscience:17-0454-81 | used at 1:400 |
| Antibody | anti-CD8a | BD Pharmingen |
BD Pharmingen:553030 | used at 1:200 |
| Antibody | anti-CD3 | BD Pharmingen |
BD Pharmingen:553067 | used at 1:400 |
| Antibody | anti-CD44 | Biolegend | Biolegend:103012 | used at 1:400 |
| Other | H-2Kb SIINFEKL tetramer |
NIH Tetramer Core |
NIH Tetramer Core:K(b)/Ova.SIINFEKL |
used at 10 nM |
| Other | H-2Kb SIYRYYGL tetramer |
NIH Tetramer Core |
NIH Tetramer Core:K(b)/SIYRYYGL |
used at 10 nM |
| Other | H-2Kb MSIIFFLPL tetramer |
NIH Tetramer Core |
NIH Tetramer Core:custom |
used at 10 nM |
| Other | H-2Kb SNFVFAGI tetramer |
NIH Tetramer Core |
NIH Tetramer Core:K(b)/mp68.SNFVFAGI |
used at 10 nM |
| Other | H-2Kb VTFVFAGL tetramer |
NIH Tetramer Core |
NIH Tetramer Core:custom |
used at 10 nM |