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. 2018 May 31;38(3):BSR20180100. doi: 10.1042/BSR20180100

Figure 1. Nicotine increases mesangial cell proliferation in high glucose milieu.

Figure 1

Human mesangial cells were cultured in serum-free medium with 5 mM glucose for 24 h, and then were incubated in medium containing nicotine (10 μM) and high glucose (30 mM) for 72 h. Nuclei were stained with Hoechest 33342, and cell numbers were counted under microscope (A,B). Representative microphotographs are displayed to show cell growth (A). The cell numbers were calculated from three independent experiments (B). Cells were immunolabeled for Ki-67; representative microfluorographs are displayed (C,D). Ki-67 positive cells are indicated by arrows (C). Ki-67 positive cell ratios were calculated from three independent experiments (D). *P<0.05 in comparison with control (5 mM glucose, 0 μM nicotine) and #P<0.05 in comparison with nicotine treatment (5 mM glucose, 10 μM nicotine). Scale bars = 100 and 20 μm for (A,C).