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. 2018 May 31;38(3):BSR20180100. doi: 10.1042/BSR20180100

Figure 3. Nicotine activates mesangial cell Wnt/β-catenin pathway in high glucose milieu.

Figure 3

Human mesangial cells were cultured in serum-free medium with 5 mM glucose for 24 h, and then were stimulated for 24 h with nicotine (10 μM) and high glucose (30 mM). (A) RNAs were extracted and real-time PCR was performed to detect the expression of fibronectin mRNA. (B) Cell lysates were collected for Western blotting to detect the proteins of Wnt3, Wnt7B, and β-catenin. Representative gels are shown. (C) The protein bands were scanned and the acquired images were analyzed using the public domain NIH image program for data quantitation. Expression of Wnt3, Wnt7B, and β-catenin was normalized to β-actin. Data are presented as fold of control expression. (D) The mediums were collected for ELISA to determine the secretion of Wnt3. *P<0.05 in comparison with control (5 mM glucose, 0 μM nicotine), and #P<0.05 in comparison with nicotine treatment (5 mM glucose, 10 μM nicotine).