Figure 1.
EMC Is Required for Optimal β1AR Biogenesis in Cells
(A) Diagram and topology of constructs for analysis of protein biogenesis by flow cytometry. All constructs contain GFP and RFP separated by a viral 2A peptide that mediates peptide bond skipping. Changes in the stability of a test protein fused to one of the fluorescent proteins changes the GFP:RFP fluorescence ratio.
(B) Histograms of flow cytometry data monitoring the fluorescence protein ratio in the indicated U2OS cell lines for each construct. “ΔEMC5” indicates a knockout of EMC5, while “rescue” indicates ΔEMC5 cells rescued by inducible re-expression of a stably integrated EMC5.
See also Figure S1.