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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: Curr Opin Cell Biol. 2018 Jul 19;55:87–95. doi: 10.1016/j.ceb.2018.06.012

Figure 1. Next-generation technologies, often used in combination or complementation, advance insights into SC niche complexities.

Figure 1

(a) Molecular profiling by population-based RNA sequencing and by single cell RNA sequencing reveal unprecedented level of gene expression complexities and cell heterogeneity in SCs and their niches. (b) In-vitro cultures with 2D engineered matrices and co-culture, 3D spheroid aggregates, and structured 3D organoids enable modeling of SC interactions with their niches. (c) 3D multicolor light microscopy of 3D cleared tissues and 4D intravital and in-vitro live imaging allow observation of SC niche complexities in vivo and in real-time.