FIGURE 1: HuRI-generated protein-protein interaction networks for IFT-B components.

The Human Reference Protein Interactome (HuRI) Mapping Project (http://interactome.baderlab.org/) identifies binary protein-protein interactions using yeast two-hybrid as the primary screening method. For the interaction networks, the red circles denote the analyzed IFT-B members while the blue circles indicate interacting proteins. Interactors with known ciliary associations are denoted with a yellow star. Interactions are characterized with different colored lines indicating variable levels of validation: green lines (published interaction; at least two pieces of experimental evidence of which at least one stems from a binary interaction detection assay), orange lines (validated interaction; interactions that are not yet published but originate from datasets that have been validated by successfully testing a representative subset of these interactions in at least one orthogonal binary interaction detection assay), red lines (verified interaction; interactions that are neither published nor validated but were identified in pooled yeast two-hybrid (Y2H) screens, tested positive in Y2H pairwise test and sequence confirmed), or blue lines (interaction found in literature). The thickness of the line indicates the strength of the interaction between two proteins. Dashed purple ellipses indicate shared interactors between IFT-B members.