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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Parasitol Res. 2018 May 30;117(8):2457–2466. doi: 10.1007/s00436-018-5934-3

Figure 2.

Figure 2

a. SRS13 does not influence parasite growth under either tachyzoite or bradyzoite conditions. The number of parasites per vacuole were counted at 36 hours after infection either in tachyzoite medium (pH7) or bradyzoite medium (pH8). At least 100 vacuoles were counted for each strain of parasite. Bars represent SD values; * represents statistical significance (*p <0.5, **p <0.01, multiple t test).

b. Immunoblot image of parasite lysate with SalmonE. This demonstrates that SRS13 (150 kDa band) protein is upregulated in bradyzoites (pH8 medium). TgALD1 was used for parasite loading control. Figure 1a is a part of this membrane.

c. SRS13 does not influence in vitro differentiation efficiency. The graph shows the rate of bradyzoite specific GFP positive vacuoles in differentiation medium at 72 hours after infection. The bars represent SD values. There was no statistically significant difference between WT and Δsrs13.