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. 2018 Jul 19;103(12):1969–1979. doi: 10.3324/haematol.2018.190652

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Figure 2. — Twist1 deletion in the bone marrow microenvironment changes expression of niche factors. (A) Quantitative real-time polymerase chain reaction (qRT-PCR) analysis of the expression of C-X-C motif chemokine ligand 12 (Cxcl12), Vascular cell adhesion molecule 1 (Vcam1), Stem cell factor (Scf) and Angiopoietin-1 (Angpt1) in freshly sorted mesenchymal stem cells (MSC), osteolineage cells (OLC) and endothelial cells (EC) from chimeric control (Ctrl) and knockout (KO) mice (n=4). (B) qRT-PCR analysis of the expression of Osteopontin (Opn) in MSC and OLC from chimeric Ctrl and KO mice (n=4). (C) Enzyme-linked immunosorbent assay analysis of BM protein concentrations of CXCL12, VCAM1, SCF and OPN in chimeric Ctrl and KO mice (n=5-8). Column plots show the mean ± standard deviation from three independent experiments. ^*P<0.05; ^**P<0.01; ^***P<0.001 (Student t test).