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. 2018 Jul 19;103(12):1969–1979. doi: 10.3324/haematol.2018.190652

Figure 4.

Figure 4.

Twist1 deletion causes impaired quiescence, self-renewal of hematopoietic stem cells and myeloid skewing. (A) Percentage of the cell cycle distribution of CD34−LSK (Lin−Sca-1+c-Kit+) cells in chimeric control (Ctrl) and knockout (KO) mice. Representative flow activated cell sorting profiles are shown on the left, and cell frequency is shown on the right (n=4-5, two independent experiments). (B) Proliferation analysis of CD34−LSK cells in chimeric Ctrl and KO mice (n=4-5, two independent experiments). (C) Schematic overview of the serial transplantation assay. (D) Percentages of donor-derived peripheral blood (PB) cells and bone marrow (BM) cells after the primary and secondary competitive transplants (n=5, two independent experiments). (E-F) Frequency (E) and number (F) of common myeloid progenitors (CMP, CD34+CD16/32−Lin−Sca-1−c-Kit+), granulocyte/macrophage progenitors (GMP, CD34+CD16/32+Lin−Sca-1−c-Kit+), megakaryocyte/erythroid progenitors (MEP, CD34−CD16/32−Lin−Sca-1−c-Kit+) and common lymphoid progenitors (CLP, Lin−Sca-1lowc-KitlowIL7R+) in chimeric Ctrl and KO mice (n=4-5, three independent experiments). (G-H) Frequency (G) and number (H) of B cells (B220+), T cells (CD3+), myeloid cells (Mac-1+ and Gr-1+) and erythrocytes (Ter119+) in chimeric Ctrl and KO mice (n=4-5, three independent experiments). Column plots show the mean ± standard deviation. *P<0.05; **P<0.01; ***P< 0.001 (Student t test).