Figure 4.

miR-150 regulation by MYC in leukemic blasts. MYC occupancy at putative regulatory loci of the MIR150 gene in (A) K562, (B) KCL-22 and (C) HL-60 cells with or without imatinib treatment. Columns represent the fold change (FC) in the % of DNA input obtained in the control (gray) or imatinib-treated (dark) cells compared with the non-specific IgG precipitation and equalized to 1 (dashed line). The promoter region of the miR-15a/16-1 cluster was used as the positive control of MYC DNA binding. Unpaired two-tailed Student t-test was used to determine P-values. ^*P<0.05, ^**P<0.01. Error bars represent standard deviations.