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Purification of a MtL and b TtL. Lane M, protein marker; lane 1, crude culture filtrate; lane 2, laccase purified by hydrophobic chromatography (HiTrap™ Phenyl FF) and concentrated for the next purification step; lane 3, laccase purified by gel chromatography (Superdex™ 75 10/300 GL). Protein fractions were separated on 12% polyacrylamide gels and stained using Instant Blue™ solution
