Table 1.

Advantages and dis-advantages of pipette tip assay (s-ARU) method

Advantages of pipette tip assay (s-ARU method)	Disadvantages of pipette tip assay (s-ARU method)
It can be used for adherent and semi-adherent cell lines	The use of pipette tips for building the modified cover plate unit is prone to human errors
Effective for carrying out toxicity test, drug assay and migration assay	It is time consuming (in the case of semi-adherent cells) when having multiple samples or compounds for analysis
It can be utilized for different microbiological and pathological applications (e.g. testing bioactive compounds against bacterial strains)	In case of semi-adherent cell lines, it is difficult to carry out time-lapse studies with the single plate and needs multiple units to be performed
As compared with the available methods, this method is consistent, reliable and effective	Due to improper operator handling, contamination can be a prime factor affecting final results
It is economical, user friendly and cost effective	Prototype is yet to be established to fulfill all the standards required for global acceptance and commercial production