Table 3.

Comparative analysis of different migration assays with respect to pipette tip assay (s-ARU method)

Cell migration methods/techniques/assays	Pipette tip assay (s-ARU method)	Trans-well migration assay (Boyden chamber)	Scratch Assay	Electric fence cell migration assay or ECIS	Spheroid migration assay	Capillary tube assays	Time-lapse cell tracking
Cell types supported	Adherent and semi-adherent cell cultures	Adherent, semi-adherent and non-adherent cell cultures	Adherent cell cultures	Adherent cell cultures	Adherent cell cultures	Adherent cell cultures	Adherent cell cultures
Measurement	Area or diameter	Number of cells	Area	Area	Migration area	Migration tube distance	Cell migration path
Complexity	Extremely simple	Extremely simple	Extremely simple	Simple	Simple	Simple	Simple
Cost	Low	High	Low	High	High	High	High
References	–	Kramer et al. (2013) and Poujade et al. (2007)	Kramer et al. (2013), Liang et al. (2007) and Geback et al. (2009)	Kramer et al. (2013) and Cai et al. (2000)	Kramer et al. (2013) and Knupfer et al. (2001)	Kramer et al. (2013) and Young et al. (1999)	Kramer et al. (2013) and Zantl and Horn (2011)