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. 2018 Nov 30;8:17485. doi: 10.1038/s41598-018-35702-y

Figure 2.

Figure 2

L. reuteri HCM2 preserves intestinal morphology in ETEC infected mice. (A) The load of ETEC in the jejunal tissues at D16; (B) The load of ETEC in the jejunal contents at D16; (C) The load of ETEC in the jejunal tissues at D18; (D) The load of ETEC in the jejunal contents at D18; E to J: Representative images of HE staining of the jejunum of weanling mice are shown (×100; n = 5). The villus length and crypt depth were measured as indicated in the image in (E). The black arrows in G and I indicate jejunal tissues that were damaged by ETEC. Control: mice received a basal diet. HCM2: mice received a basal diet and 109 CFUs L. reuteri HCM2 daily for two consecutive weeks. ED1: mice received a basal diet, were challenged with 108 CFUs ETEC by intragastric administration at day 15, then received a basal diet for 1 day. HED1: mice received a basal diet and 109 CFUs L. reuteri HCM2 daily for two consecutive weeks, were challenged with 108 CFUs ETEC by intragastric administration at day 15, and then received a basal diet for 1 day. ED3: mice received a basal diet, were challenged with 108 CFUs ETEC by intragastric administration at day 15, then received a basal diet for 3 days. HED3: mice received a basal diet and 109 CFUs L. reuteri HCM2 daily for two consecutive weeks, were challenged with 108 CFUs ETEC by intragastric administration at day 15, and then received a basal diet for 3 days.