Table 2.
Enzymes utilized in ASAP-cloning for multiplex GEC integration into indicated vectors.
| Vector | Enzyme cutting inserts | Enzyme cutting vector | Enzyme cutting concatemers (Isocaudomers) |
|---|---|---|---|
| pX330 | BbsI | XbaI | NheI |
| pcDNA-dCas9-VP64 | BsmBI | AvaI | AgeI |
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