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. 2018 Nov 30;9:5104. doi: 10.1038/s41467-018-07639-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Eccentric contraction-induced force loss in mdx muscle recovers rapidly and is partially protected by overexpression of γ_cyto-actin, but not α_cardiac-actin. a Recovery of isometric force production in isolated extensor digitorum longus (EDL) muscles from mdx mice subjected to 10 maximal isometric or eccentric contractions. Values are expressed as a percentage of the isometric force measured before the 10 contractions (Pre) for each timepoint listed; n = 4 for both conditions. *P < 0.05, ***P < 0.001 compared with Post 0’; two-way ANOVA. b Increasing the time interval between eccentric contractions from 3 to 30 min significantly diminishes the measured force loss in EDL muscles from mdx mice; n = 4 for both conditions. *P < 0.05, **P < 0.01, ***P < 0.001 compared to 3 min Rest; two-way ANOVA. c Immunoblot analysis of γ_cyto-actin in tibialis anterior (TA), extensor digitorum longus (EDL), gastrocnemius (Gastroc), and soleus muscles from mdx/Actg1-TG mice versus non-transgenic mdx littermates. d Immunofluorescence analysis of γ_cyto-actin (green), laminin (red), and DAPI (blue) in 10 µm cryosections of quadriceps muscle from mdx/Actg1-TG mice versus non-transgenic mdx littermates. e EDL muscles isolated from mdx/Actg1-TG mice and non-transgenic mdx littermates were subjected to 10 eccentric contractions and the force measured at each contraction expressed as a percentage of the force produced during the first contraction; n = 4 for both genotypes. *P < 0.05, **P < 0.01, ***P < 0.001 compared to mdx; two-way ANOVA. f Immunoblot analysis of α_ca-actin in tibialis anterior (TA), extensor digitorum longus (EDL), gastrocnemius (Gastroc), and soleus muscles from mdx/Coco mice versus non-transgenic mdx littermates. g Immunofluorescence analysis of α_ca-actin (green), laminin (red), and DAPI (blue) in 10 µm cryosections of quadriceps muscle from mdx/Coco mice versus non-transgenic mdx littermates. h EDL muscles isolated from mdx/Coco mice and non-transgenic mdx littermates were subjected to 10 eccentric contractions and the force measured at each contraction expressed as a percentage of the force produced during the first contraction; n = 4 for both genotypes. Throughout, error bars represent means ± SEM