Fig. 3.

Genetic ablation of myoglobin partially protects mdx muscle from eccentric contraction-induced force loss. a Immunoblot analysis demonstrated the absence of myoglobin in mdx/mb^–/– muscle, that myoglobin levels are decreased in mdx, and that myoglobin levels are partially restored in mdx/p47^–/– gastrocnemius muscle; n = 4 for each genotype. *P < 0.05, **P < 0.01, ***P < 0.001; one-way ANOVA. b EDL muscles isolated from mdx and mdx/mb^–/– mice, or mdx muscles treated with 20 mM N-acetylcysteine (NAC) were subjected to 10 eccentric contractions and the forces measured expressed as a percentage of the force generated during the first eccentric contraction; n = 4 for WT and mdx+NAC; n = 9 for mdx/mb^–/–; *mdx+NAC significantly different from mdx (P ≤ 0.05); ^#mdx/mb^–/– significantly different from mdx (P ≤ 0.05); two-way ANOVA. Throughout, error bars represent means ± SEM