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. 2018 Nov 30;9:5104. doi: 10.1038/s41467-018-07639-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Cysteine 272 of γ_cyto-actin is necessary for protection of mdx muscle from eccentric contraction-induced force loss. a Immunoblot comparison of γ_cyto (Actg1-TG), γ_cyto^C272A (C272A-TG), and β_cyto (Actb-TG) overexpression in mdx gastrocnemius muscle. b Immunofluorescence analysis demonstrates similar distributions of γ_cyto, γ_cyto^C272A, and β_cyto in 10 µm quadriceps cryosections. Scale bar = 50 µm. c EDL muscles isolated from WT, mdx, mdx/Actg1-TG, mdx/C272A-TG, and mdx/Actb-TG mice were subjected to 10 eccentric contractions and the forces measured expressed as a percentage of the force generated during the first eccentric contraction; n = 8 for WT and mdx/C272A-TG; n = 5 for mdx; n = 6 for mdx/Actg1-TG and mdx/Actb-TG. *The mdx/Actg1-TG significantly different from mdx (P ≤ 0.05), ^#mdx/Actb-TG significantly different from mdx (P ≤ 0.05); two-way ANOVA. d Rate of DCF fluorescence in single flexor digitorum brevis (FDB) muscles from WT, mdx, mdx/Actg1-TG, mdx/C272A-TG, and mdx/Actb-TG mice exposed to cyclic stretch in the presence of DMSO (vehicle) or the NOX2 inhibitor gp91ds-tat; n = 9 for WT, mdx/Actg1-TG, and mdx/Actb-TG; n = 12 for mdx; n = 7 for mdx/C272A-TG. ***P < 0.001, ns no significance compared to WT; one-way ANOVA. Throughout, error bars represent means ± SEM