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. 2018 Nov 30;9:5082. doi: 10.1038/s41467-018-07474-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Robo genes preserve pancreatic progenitor identity. a Representative IF images of E9.5 CTRL and Robo1/2 KO cryosections stained for Pdx1, Prox1, Sox9 and E-cadherin (Ecad). Consecutive liver (lv) and ventral pancreas (vp’, vp”) sections are shown. Liver (lv) cells are Prox1-positive (⁺), pancreatic progenitors (vp) are Prox1⁺/Pdx1⁺. Arrowheads indicate cells shown in white insets. Scale bars, 50 µm. b Single-cell measurement of fluorescence intensity of Prox1 and Pdx1 in liver buds (grey circle) and ventral pancreas of E9.5 CTRL (light green circle) and Robo1/2 KO (dark green circle) embryos. Diamond shapes indicate Robo1/2 KO ventral pancreatic cells that appear segregated from the surrounding epithelium and overlay with lv cells (grey). Fluorescence intensity was measured with Fiji and values corrected by linear normalisation within each embryo. FI fluorescence intensity, AU arbitrary units; n = 3 per genotype