Fig. 3.

Mitochondrial capacity aids ROS production in low glycolysis. a Area under the curve (AUC) oxygen consumption rate (OCR) (total oxygen consumption in pmol) of respiratory burst in bone marrow- and spleen-derived C57BL/6J neutrophils following stimulation with phorbol 12-myristate 13-acetate (PMA, 1 µg/ml) in 0.25–25 mM glucose or following 2-deoxy glucose (2DG, 100 mM). Data (n = 5 per group) were analysed by paired one-way ANOVA with Dunnett’s multiple comparison tests indicated, data represents two similar experiments. b Representative OCR traces from C57BL/6 bone marrow neutrophil subsets following stimulation with PMA (1 µg/ml) (arrow) in the presence or absence of 2DG (100 mM) (dotted line). c Relative AUC OCR in neutrophil subsets from (b) following 2DG and PMA stimulation, when compared to PMA alone. Data (n = 3 per group) were analysed by paired t-test, from two independent experiments. p Values *<0.05, ***<0.001. All error bars display mean ± SEM