Fig. 7.

Cancer patient neutrophils possess increased OXPHOS. a Representative flow cytometry plots of peripheral blood neutrophils displaying CD10 and CD16 expression from healthy controls and ovarian cancer patients. The box indicates mature neutrophils. b Quantification of immature neutrophils from multiple patients scored as a percentage of CD14⁻ CD15⁺ cells which do not express the CD10^hi CD16^hi marker profile associated with maturity. Data were analysed by unpaired t-test control n = 3, cancer patients n = 4. c Representative flow cytometry plot (left) of peripheral human neutrophils' CD10 expression and mitotracker green fluorescence. The box indicates immature CD10^mid neutrophils. Quantification (right) of mitotracker median fluorescent intensity (MFI) from CD10 mid and hi peripheral neutrophils. Data (n = 4) were analysed by paired t-test. d ATP synthase dependent oxygen consumption rates (OCR) of peripheral blood neutrophils from healthy controls and ovarian cancer patients. Data from four independent experiments (healthy controls n = 11 and cancer patients n = 10). Data were analysed by two-way ANOVA with significance between controls and patients indicated on graph. p Values *<0.05, **<0.01. All panels error bars display mean ± SEM