Fig. 5.

FtsN and ZipA are required for preseptal peptidoglycan synthesis. a Ten-fold serial dilutions spotted plate of the parental strain WM2935 (ftsA^E124A), MPW22 (ftsA^E124A ΔzipA/rep^ts zipA), MPW23 (ftsA^E124A ΔftsN/rep^ts ftsN) and MPW29 (ftsA^E124A ΔzipA ΔftsN//rep^ts zipA ftsN) strain cultures incubated at 30 °C (permissive) and 42 °C (non-permissive). b Representative growth curves of strains under permissive (open symbols) and non-permissive temperature (close symbols). Cell culture optical density (578 nm) was kept below 0.4 during the whole experiment. c Quantification of preseptal peptidoglycan synthesis (PIPS) bands per unit of cell length in the mentioned strains under non-permissive conditions (42 °C). d–g Phase contrast and HADA fluorescent images for detection of preseptal PG synthesis in WM2935 (d), MPW22 (e), MPW23 (f) and MPW29 (g) cells under non-permissive conditions (42 °C). The non-fluorescently labelled preseptal PG synthesis bands are indicated by yellow arrows. Scale bars represent 5 µm