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. 2018 Nov 30;8:17510. doi: 10.1038/s41598-018-35552-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic figure of placement ROIs. Two subchondral bone ROIs (93 × 40 pixels, white rectangles) were placed under the cartilage-bone interface in the middle part of medial and lateral condyles of the tibia. Two trabecular bone ROIs (93 × 93 pixels, black rectangles) were placed below and parallel to the subchondral bone ROIs of the tibia. Minimum JSWs (white dotted line) were measured from the narrowest point of the joint from both the medial and the lateral sides. Anatomical angles were medially measured from the intersection of a line from the center of the head of femur to the center of the tibial spines, and a second line from the center of the tibia to the center of the tibial spines.