Fig. 1. Sequence analysis of MdFRK1/2.

a Schematic diagram of gene positions and structure of MdFRK2. Conserved regions are indicated with (a1), (a2), (a3) and (b). b Alignment of conserved regions in deduced amino acid sequences of MdFRK1/2 with other plant fructokinases. (a1–a3), 3 signature patterns for phosphofructokinase B (PfkB) family; (b), region specific to fructokinase. Black boxes indicate proteins with >50% identical amino acids. c Maximum likelihood phylogeny of MdFRK1/2 deduced amino acid sequences and those from Arabidopsis, Lycopersicon esculentum, and Solanum tuberosum. d Patterns of mRNA relative expression for MdFRK1 and MdFRK2 in developing fruit and different tissues. S, shoot tips; M, mature leaves; DAB, days after bloom