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. 2018 Nov 30;8:17514. doi: 10.1038/s41598-018-35746-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phenotypic analysis of homozygous mutants. (A) Electrophoresis of PCR genotyping. Genotypes and karyotypes are shown at the top. Primer sets are indicated to the left. Positions of Xist and Zfy PCR products are indicated with arrowheads at the right. Sizes of DNA markers are shown on the right. NC: negative control. Full agarose gel images of left and right part of (A) are shown in Supplementary Figs S2 and S3, respectively. (B) Sequences of deleted alleles are shown with electropherograms. Genotypes are indicated at top left. Positions of deleted sequences are indicated with dotted lines. The deleted lengths are shown between dotted lines. (C) Phenotype of mutant mice. Genotypes and karyotypes are shown at top. Testis and ovary are indicated with arrowheads labeled T and O, respectively.