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. 2018 Nov 30;8:17522. doi: 10.1038/s41598-018-35725-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phosphorylation of NADPH oxidase p47^phox requires both Ca²⁺ influx and ligand binding. Scale bar = 5 µm. (a) Increased phosphorylation of p47^phox at Ser328 occurs after incubation with NMDA or NMDA + 7CK + ionomycin, but not after incubation with NMDA + AP5 + ionomycin or with ionomycin in the absence of NMDA. Conditions are as in Fig. 3. (b) Quantification of results shown in (a), with data in each of n = 3 experiments normalized to the control condition; *p < 0.05 vs control.