Table 1.
KRAS copy number and mutant alleles in digested and undigested cell line DNA determined by droplet digital PCR Table 1.
| Cell line | Digested treatment | Undigested treatment | P value1 | Ratio 2 | ||||
|---|---|---|---|---|---|---|---|---|
| hTERT (copy/uL) | KRAS (copy/uL) | MU/(MU + WT) % | hTERT (copy/uL) | KRAS (copy/uL) | MU/(MU + WT) % | |||
| RPMI-8226 | 17101 ± 174 | 17473 ± 274 | 66.61 ± 0.55 | 15186 ± 750 | 10985 ± 31 | 66.76 ± 1.17 | 6.93E-5 | 1.02 |
| SNU-C2B | 17401 ± 194 | 16451 ± 358 | 48.68 ± 0.29 | 14114 ± 705 | 13773 ± 175 | 47.89 ± 0.72 | 2.49E-3 | 0.95 |
| NCI-157 | 15724 ± 530 | 27475 ± 525 | 51.32 ± 0.61 | 13901 ± 695 | 19753 ± 160 | 51.84 ± 0.09 | 3.04E-4 | 1.75 |
| SW1573 | 16278 ± 244 | 16178 ± 210 | 99.92 ± 0.05 | 14125 ± 706 | 6184 ± 26 | 99.88 ± 0.05 | 1.84E-3 | 0.99 |
| A549 | 20330 ± 686 | 21259 ± 201 | 99.99 ± 0.07 | 111819 ± 590 | 16498 ± 440 | 99.96 ± 0.02 | 4.26E-4 | 1.05 |
| SW620 | 18840 ± 640 | 54201 ± 479 | 100.00 ± 0.18 | 16828 ± 841 | 42100 ± 845 | 99.97 ± 0.06 | 2.30E-4 | 2.88 |
| HCT116 | 16286 ± 124 | 16049 ± 174 | 49.99 ± 0.04 | 12844 ± 642 | 15290 ± 15 | 50.36 ± 0.56 | 0.01 | 0.99 |
| 293 T | 18748 ± 104 | 18641 ± 204 | — | 11572 ± 549 | 15682 ± 308 | — | 1.12E-3 | 0.99 |
KRAS copy number and mutant alleles in digested and undigested cell line DNA determined by droplet digital PCR.
1P value of T test for KRAS copy number between digested and undigested treatment.
2The ratio of KRAS copy number to hTERT copy number.