Table 2.

Sequencing noise for KRAS mutation by next generation sequencing.

Mutation		Replicates
		1	2	3	4	5	6
G12S	Noise level	0.04%	0.03%	0.03%	0.03%	0.03%	0.03%
	Sequencing Depth	198412	179009	211438	42934	44453	41823
G12R	Noise level	0.01%	0.01%	0.01%	0.01%	0.02%	0.02%
	Sequencing Depth	198412	179009	211438	42934	44453	41823
G12C	Noise level	0.03%	0.20%	0.20%	0.22%	0.18%	0.23%
	Sequencing Depth	198412	179009	211438	42934	44453	41823
G12V	Noise level	0.48%	0.37%	0.33%	0.40%	0.32%	0.39%
	Sequencing Depth	198894	179017	211446	42932	44462	41830
G12D	Noise level	0.01%	0.01%	0.01%	0.02%	0.02%	0.01%
	Sequencing Depth	198894	179017	211446	42932	44462	41830
G12A	Noise level	0.09%	0.07%	0.06%	0.08%	0.04%	0.04%
	Sequencing Depth	198894	179017	211446	42932	44462	41830
G13D	Noise level	0.02%	0.02%	0.02%	0.01%	0.02%	0.01%
	Sequencing Depth	198878	179005	211425	42934	44469	41818