Table 3.
KRAS mutant alleles in serial dilution determined by next generation sequencing.
| Dilution | Mutant allele percentage prepared by gravimetrical (%) | Sequence coverage | Mutant allele percentage determined by NGS (%) |
|---|---|---|---|
| G12A-S4 | 0.98 | 8684.19 | 0.89% |
| G12A-S5 | 0.19 | 8097.67 | 0.16 |
| G12A-S6 | 0.10 | 7968.80 | ND |
| G12D-S1 | 21.29 | 1075.02 | 20.00 |
| G12D-S2 | 10.75 | 966.58 | 10.10 |
| G12D-S3 | 5.41 | 1135.16 | 5.20 |
| G12D-S4 | 1.06 | 8899.51 | 1.10 |
| G12D-S5 | 0.10 | 9986.04 | 0.21 |
| G12D-S7 | 0.01 | 8703.11 | ND |
| G12C-S4 | 0.97 | 9510.11 | 1.0% |
| G12C-S6 | 0.06 | 8857.25 | ND |
| G12C-S7 | 0.01 | 9268.47 | ND |
| G12S-S4 | 1.1 | 8426.12 | 0.69 |
| G12S-S5 | 0.09 | 8283.83 | 0.15 |
| G12V-S0 | 59.96 | 950.43 | 61.80 |
| G12V-S1 | 27.27 | 1091.55 | 25.90 |
| G12V-S2 | 14.22 | 1133.44 | 14.70 |
| G12V-S3 | 7.23 | 953.18 | 6.10 |
| G12V-S4 | 1.53 | 9214.20 | 1.40 |
| G12V-S5 | 0.17 | 7547.37 | ND |