Figure 4.

Relative expression of intracellular miRNA let-7d in different experimental groups. (A) MKN-45 cells (NCL⁺) were treated with 200nM conjugate-1, 250nM conjugate-2, 200 nM mixture of aptNCL plus miRNA let-7d and control-oligonucleotides or (B) transfected with 200 nM miRNA let-7d and control-miRNA. After 24 and 48 h, relative miRNA let-7d levels were quantified by qRT-PCR. (C) HDF cells (NCL^-) were treated or (D) transfected with the indicated concentrations of the oligonucleotides. After 48 h, relative miRNA let-7d levels were assessed by qRT-PCR. (E) Comparison of intracellular let7d between MKN-45 and HDF cell lines under similar treatments. * P < 0.0001 by comparison with the untreated control cells;   P < 0.0001 by comparison with the conjugate-1 and conjugate-2; ^&P < 0.0001 by comparison with HDF cells. Data are presented as mean ± SD. Ctrl, Control; Conj, Conjugate; Transf, Transfected; aptNCL, nucleolin aptamer