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. 2018 Nov 30;8:17498. doi: 10.1038/s41598-018-35316-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) 16-ch BLM chip and magnified images of the double well. The double well was micromachined on an acrylic basement and the separator was inserted in its middle. Ag/AgCl electrodes were placed at the bottom of the well. (b) Spring contact probes integrated into the mount system. (c) Photograph of 16-ch BLM chip equipped on the mount system. (d) Probes were pressed to each electrode from the bottom, and provided reliable electrical connection between the electrodes and the amplifier due to the spring. (e) Current traces of α-hemolysin nanopore using our system. Applied potential: +100 mV.