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. 2018 Nov 30;8:17498. doi: 10.1038/s41598-018-35316-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Current traces of the TRPML1 channel. Applied potential: +80 mV, −60 mV, and −80 mV. (b) Inhibition of TRPML1 channel signal by the TRPML1 inhibitor, verapamil. The arrow represents the addition of verapamil. The final concentration of verapamil was 1 mM. Applied potential: +80 mV. (c) Current trace of the RyR channel. Applied potential: +40 mV and +80 mV. (d) Inhibition of the RyR channel’s signal by the RyR inhibitor, ruthenium red. The arrow represents the addition of ruthenium red. The final concentration of ruthenium red was 500 nM. Applied potential: +40 mV.