Table 1.
Buffer solution and inhibitor conditions for respective ion channel recordings.
| Ion channel | hERG | TRPV1 | NMDA | TRPML1 | RyR |
|---|---|---|---|---|---|
| Buffer composition of droplet A | 10 mM HEPES 120 mM KCl (pH 7.2) |
10 mM HEPES 140 mM NaCl 5 mM KCl 2 mM CaCl2 2 mM MgCl2 10 mM glucose 1 μM capsaicin (pH 7.4) |
10 mM HEPES 150 mM NaCl 2.5 mM KCl 10 mM glutamate 10 mM glycine (pH 8.0) |
10 mM HEPES 150 mM KCl (pH 7.4) |
20 mM HEPES 250 mM KCl 200 μM CaCl2 (pH 7.4) |
| Buffer composition of droplet B | 10 mM HEPES 120 mM KCl (pH 7.2) |
10 mM HEPES 140 mM KCl 5 mM EGTA (pH 7.4) |
10 mM HEPES 135 mM KCl 1 mM CaCl2 (pH 7.4) |
10 mM HEPES 150 mM KCl (pH 7.4) |
20 mM HEPES 250 mM KCl 3 mM ATP 10 μM CaCl2 4 mM caffeine (pH 7.4) |
| Addition droplet of crude membrane fraction | Both Droplet | Droplet B | Droplet A | Droplet A | Droplet B |
| Inhibitor concentration and droplet topology | 100 nM astemizole in Droplet B 200 μM E-4031 in both droplets |
100 μM capsazepine in Droplet A | 100 μM MK-801 in Droplet A 5 mM MgCl2 in both droplets |
1 mM verapamil in Droplet A | 500 nM ruthenium red in both droplets |