Table 1.

Calibration curve range, FA retention time, selected MS ion fragment, limit of detection (LOD) in femtograms (fg) on-column (OC), and lower limit of quantitation (LLOQ) in picograms (pg) for each of the quantified FAs. Trans- FAs found in plasma were assumed to have the same instrumental response as their commercially available cis- counterparts.

Fatty Acid	Calibration Curve Range in pg on column; (in ng/mL)	Retention Time (min)	SRM Mass Transition	LOD in fg OC;	LLOQ in pg OC; (in ng/mL)
	0–469;				4.3;
5,6-DHET	(0–20)	5.8	505→239	25	(0.185)
	0–469;				4.3;
8,9-DHET	(0–20)	4.8	505→239	100	(0.185)
	0–469;				4.3;
11,12-DHET	(0–20)	4.0	505→239	100	(0.185)
	0–469;				4.3;
14,15-DHET	(0–20)	3.4	505→239	50	(0.185)
	0–2340				26;
5-HETE	(0–100)	9.8	487→283	50	(1.11)
	0–2340;				26;
9-HETE	(0–100)	8.7	487→307	50	(1.11)
	0–2340;				26;
11-HETE	(0–100)	7.6	487→335	50	(1.11)
	0–2340;				26;
12-HETE	(0–100)	7.9	487→347	50	(1.11)
	0–2340;				26;
15-HETE	(0–100)	6.9	487→183	10	(1.11)
	0–350;s				1.7;
19-HETE	(0–15)	4.8	487→183	50	(0.074)
	0–350;				1.7;
20-HETE	(0–15)	5.2	487→183	50	(0.074)
	0–700;				4.0;
5,6-EET	(0–30)	11.6	487→283	25	(0.185)
	0–700;				4.0;
8,9-EET	(0–30)	10.8	487→307	25	(0.185)
	0–700;				4.0;
11,12-EET	(0–30)	10.3	487→333	25	(0.185)
	0–700;				4.0;
14, 15-EET	(0–30)	9.0	487→333	75	(0.185)