Table 3.
Comparing hydrolysis efficiency and reproducibility of FAs in pooled plasma using PLA2 to KOH. Column 1 compares the peak area ratios of the FAs when hydrolyzed by PLA2, divided by the peak area ratio of that fatty acid hydrolyzed by KOH (PLA2 treated plasma FA Peak area/IS Peak area)/(KOH treated plasma FA peak area/IS Peak Area). Numbers below 100% signify more FA was observed using KOH, while numbers above 100% signify more FA was observed by PLA2 hydrolysis. For all FAs with the exception of 19-HETE, more FAs were observed with KOH. The greater quantity of observed FAs from KOH may be due to the less specific nature of KOH hydrolysis. Columns 2 and 3 are the CV for PLA2 and KOH hydrolysis, respectively.
| PLA2/KOH (%) | PLA2 CV (%) | KOH CV (%) | |
|---|---|---|---|
| 5,6-DHET | 14.6 | 32.8 | 18.9 |
| 8,9-DHET | 44.7 | 18.5 | 17.5 |
| 11,12-DHET | 22.4 | 16.7 | 29.6 |
| 14,15-DHET | 51.0 | 60.0 | 37.7 |
| 5-HETE | 18.3 | 18.0 | 14.5 |
| 8-HETE | 37.7 | 9.0 | 5.5 |
| 9-HETE | 31.1 | 7.4 | 6.9 |
| 11-HETE | 34.1 | 9.9 | 8.3 |
| 12-HETE | 65.3 | 5.7 | 4.5 |
| 15-HETE | 45.6 | 18.5 | 11.7 |
| 19-HETE | 142.4 | 26.1 | 10.6 |
| 20-HETE | 89.4 | 8.7 | 9.8 |
| 5,6-cis-EET | 80.1 | 38.6 | 21.2 |
| 5,6-trans-EET | 68.9 | 59.8 | 24.2 |
| 8,9-cis-EET | 51.7 | 31.6 | 21.0 |
| 8,9-trans-EET | 39.3 | 61.0 | 16.9 |
| 11,12-cis-EET | 37.0 | 33.0 | 21.4 |
| 11,12-trans-EET | 37.4 | 56.8 | 11.4 |
| 14,15-cis-EET | 41.4 | 22.2 | 33.6 |
| 14,15-trans-EET | 36.7 | 50.6 | 14.3 |
| 14,15-DHET-d11 | 95.9 | 8.5 | 6.5 |
| 12-HETE-d8 | 97.3 | 11.4 | 5.3 |
| 5,6-EET-d8 | 88.1 | 6.3 | 27.3 |
| 8,9-EET-d8 | 116.3 | 5.6 | 9.4 |
| 14,15-EET-d8 | 143.8 | 6.1 | 11.2 |