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Effect of AMEE on RANKL-induced osteoclast differentiation in BMMs. BMMs were cultured with vehicle or AMEE in the presence of M-CSF (60 ng/mL) and RANKL (100 μg/mL) for 4 days. (A) TRAP staining. (B) Total cellular TRAP activity. (C) Osteoclast number. (D) Effect of AMEE on the viability of BMMs was determined by Cell Counting Kit-8 (CCK-8) assay. * p < 0.05; ** p < 0.01; *** p < 0.001 versus vehicle-treated control.
