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Effect of AMEE on RANKL-induced c-Fos and NFATc1 expression in BMMs. BMMs were cultured with vehicle or AMEE (100 μg/mL) in the presence of M-CSF (60 ng/mL) and RANKL (100 μg/mL) for the indicated days. (A) mRNA levels of c-Fos and NFATc1 were analyzed by qPCR. ** p < 0.01; *** p < 0.001 versus vehicle-treated control. (B) Protein expression levels of c-Fos and NFATc1 were determined by Western blot analysis. Total p38 was used as a loading control.
