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Effect of AMEE on the activation of MAP kinases and NF-κB in BMMs. BMMs were pretreated with AMEE (100 μg/mL) for 3 h and then stimulated with RANKL (100 ng/mL) for the indicated times. The total and phosphorylation levels of the indicated proteins were determined by Western blot analysis. β-actin was used as a loading control.
