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. 2013 Jun 24;18(7):7376–7388. doi: 10.3390/molecules18077376

Figure 6.

Figure 6

Phytochemical profile of AMEE and the anti-osteoclastogenic effects of atractylenolides. (A) HPLC chromatograms of a standard mixture of atractylenolide I, II, and III and AMEE at 210 and 280 nm. (B) BMMs were cultured with vehicle (DMSO) or atractylenolide I (AT-I), II (AT-II), and III (AT-III) in the presence of M-CSF (60 ng/mL) and RANKL (100 μg/mL) for 4 days. TRAP-positive multinucleated cells containing more than three nuclei were counted as osteoclasts. * p < 0.05; ** p < 0.01; *** p < 0.001 versus vehicle-treated control. (C) Effects of atractylenolide I, II, and III on the viability of BMMs were determined by CCK-8 assay.