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. 2013 Jan 11;18(1):845–858. doi: 10.3390/molecules18010845

Figure 5.

Figure 5

(A) Effects of chamaejasmine on proteins expression of Bax, Bcl-2 in MDA-MB-231cells. (B) Effects of chamaejasmine on proteins expression of caspase-3 and -8 in MDA-MB-231 cells. (C) Effects of chamaejasmine on IKKα, IKKβ, phosphorylation IKBα as well as degradation of IκBα in MDA-MB-231 cells. (D) Effects of chamaejasmine on activation of NF-κB in MDA-MB-231 cells by western blotting analysis. As detailed in Materials and Methods, the cells were treated with chamaejasmine (4–16 μM) and then harvested. Total cell lysates were prepared and 20 μg proteins were subjected to SDS-PAGEfollowed by western blotting analysis. Equal loading of proteins was confirmed by strippingthe western blot and reprobing it for β-actin. The western blots shown here are representativeof three independent experiments with similar results. The values below the figures represent relative density of the bands normalized to β-actin.