Table 4.
Kinetic parameters of cholesterol synthesis enzymes with specific sterols as substrates.
| Enzyme | Substrate sterol | Vmax [nmol/min per mg of protein] | Km [µM] | kcat [Vmax/Km] | Relative kcat (fold) | Reference |
|---|---|---|---|---|---|---|
| CYP51A1 | Lanosterol | 262.3 | 10.5 | 24.1 | [25] | |
| 24,25-dihydrolanosterol | 377.4 | 20.0 | 18.87 | |||
| DHCR14 | 4,4-dimethy-5α-cholesta-7,24-dien-3β-ol | 1.13 | 29.4 | [26] | ||
| EBP | Zymosterol | 0.325 | 25 | [27] | ||
| SC5DL | Lathosterol | 0.017 | 15.4a | [28] | ||
| DHCR7 | 7-dehydrocholesterol | 0.85 | 30 | [22] | ||
| DHCR24 | Lanosterol | 0.361 | 109 | 0.0033 | 1.0 | [7] |
| Zymosterol | 3.550 | 176 | 0.0201 | 6.1 | ||
| 7-dehydrodesmosterol | 2.176 | 37 | 0.0586 | 17.8 | ||
| Desmosterol | 2.945 | 163 | 0.0180 | 5.5 |
a nmol/mg of membrane protein.